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Biochemistry
Determination of the Michaelis constant with Cobra SMARTsense
Article No:
P4120369
The enzymatic hydrolysis of urea in aqueous solution yields carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements can be used to determine the rates of urea hydrolysis by the enzyme urease at different substrate concentrations. The Michaelis constant can be calculated from these values.
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