Multicomponent analysis with spectrophotometry (mixed colour photometry)

In solutions containing different-coloured substances the concentrations of the dyes can be anlysed by spectrometry without prior separation of the substances. Using the integrated software the spectra of the pure dye solutions and mixtures thereof will be recorded. Calibration curves for each substances enable us to determine the quantity of that substance in the solution. Benefits Experimental procedure without dangerous chemicals Modern, easy-to-use photospectrometer Simple evaluation by supplied software Tasks In a mixed solution containing fuchsine acid and patent blue V, the proporion of fuchsine acid is to be determined. Learning objectives Photometry UV-VIS spectrometry Lambert-Beer's law Dyes Absorption of light Necessary accessories Precision balance 620g/0.001g Software integrated in spectrometer. Computer not provided.